B12 10MG
$39.00
B12 is cyanocobalamin, a synthetic form of vitamin B12, supplied as 10mg of lyophilized powder for laboratory research. Each lot is independently tested and published against its lot number.
- Cyanocobalamin, molecular weight 1355.4 Da
- Purity, identity and content per vial tested by HPLC on every lot
- Certificate of analysis published against every lot
- Research use only Not for human or veterinary use
- Certificate per lot Published against the lot number
- Independently tested RP-HPLC with mass spec identity
- Restricted sale 21+ qualified researchers only
What is B12?
B12 is supplied here as cyanocobalamin, a synthetic form of vitamin B12, as a lyophilized powder for laboratory research. It is a cobalt containing corrinoid used widely as a reference compound in analytical and cell culture work.
B12 structure
Cyanocobalamin has the formula C63H88CoN14O14P and a molecular weight of 1355.4 Da. It consists of a corrin ring coordinating a central cobalt ion, with a cyanide ligand in the upper axial position and a dimethylbenzimidazole nucleotide in the lower. It is light sensitive in solution and should be stored protected from light.
Where this material appears in the literature
Cyanocobalamin appears throughout in vitro literature as a cell culture media component and as a reference compound in studies of cobalamin dependent enzymes and one-carbon metabolism. Altherix Labs supplies the material for that kind of laboratory investigation and makes no claim about what it does in any living subject.
Related material in the catalogue
B12 is held under small molecule compounds alongside NAD+.
Analysis and certification
Every lot is analyzed by an independent laboratory before release, using RP-HPLC for purity and LC-MS for identity confirmation. The certificate of analysis is published against the lot number rather than against the product page, so the document you read is the document for the vial you receive. The lot number printed on the vial label is the link between the two.
Purity and content
Purity is reported as the main peak’s share of total integrated peak area by HPLC. It shows how much of the detected material is the target compound, not how much of the powder is peptide. Net content per vial is measured separately and reported on the same certificate in mg.
The measured figures for the lot you receive are stated on its certificate of analysis, and that certificate is the record that applies to your material. Analytical figures relate to the lot they are published against and carry no implication of suitability for any purpose beyond laboratory research.
Research use only
Altherix Labs supplies this material as a reference standard for laboratory research. It is not supplied for any use in humans or animals, it is not a drug, dietary supplement, food or cosmetic, and nothing on this page describes or implies any such use. Sale is restricted to persons aged 21 or over who are qualified researchers or who purchase on behalf of a research institution, laboratory or business.
| Compound | B12 |
|---|---|
| Also known as | Cyanocobalamin, vitamin B12 |
| Class | Corrinoid small molecule |
| Molecular formula | C63H88CoN14O14P |
| Molecular weight | 1355.37 Da |
| CAS number | 68-19-9 |
| Appearance | Dark red crystalline powder |
| Purity reporting | Measured on every lot, stated on the certificate of analysis |
| Purity method | RP-HPLC |
| Identity method | LC-MS |
| Solubility | Soluble in sterile water and aqueous buffers for analytical preparation |
| Labeled quantity | 10mg per vial |
Characterization. Values above describe the compound as supplied. The figures for the lot you receive, including measured purity, are stated on its certificate of analysis.
How this material is tested
Two separate analyses are carried out on every lot before release, and both appear on the certificate.
- RP-HPLC, for purity. Reversed phase high performance liquid chromatography separates the material by hydrophobicity. The main peak is integrated against total peak area, and that ratio is the purity figure reported on the certificate.
- LC-MS, for identity. Liquid chromatography coupled to mass spectrometry confirms that the mass of the main peak matches the expected mass for the compound, which is what distinguishes the correct compound from a closely related one.
A complete certificate carries the compound name, the lot number, the test date, the method, the measured purity and the name of the laboratory. If any of those is missing, or the lot number on the vial does not match the certificate, contact us before using the material.
Storing B12
On arrival, move the sealed vial into freezer storage. Store the vial dry at −20°C or below, protected from light and moisture. Returned promptly to those conditions, the dry solid is the most stable form this material takes.
Preparing stock solutions
Prepare stock solutions in sterile water or an appropriate aqueous buffer at a concentration suited to your assay, expressed in mg/mL. Keep stock solutions protected from light. Allow the vial to reach room temperature before opening, add solvent down the vial wall rather than directly onto the solid, and let the material dissolve without vigorous agitation. B12 dissolves readily in water at the concentrations used for analytical work.
Solutions are less stable than the lyophilized solid. Prepare close to the point of use, hold at 2 to 8°C for short periods only, and aliquot before freezing so that repeated freeze and thaw cycles are avoided.
On arrival
Check that the vial is intact and sealed, that the compound name and labeled quantity match your order, and that the lot number on the label matches the certificate published for it. If anything does not match, or the packaging has clearly failed in transit, contact us before using the material. Problems visible on arrival should be reported promptly so that a carrier claim remains possible.
Handling and disposal
Assess the hazards of this material before handling it and use appropriate controls and protective equipment. It has not been characterized toxicologically and should be treated as potentially hazardous. Anyone handling it should be trained for the work.
Dispose of unused material, vials and packaging lawfully and in line with your institution's waste procedures.
Record keeping
Record the lot number in your notes at the point of first use, alongside the date the vial was opened and the concentration of any stock prepared. Certificates are published against the lot number rather than the product, so the lot number is the only reliable link between your results and the analytical data for the material that produced them. If a question arises about a result months later, that link is what keeps the work auditable.
Where do I find the certificate of analysis for my vial of B12?
Certificates are published against the lot number, not the product. Find the lot number printed on the vial label and look it up on the Lab Reports page. The certificate for that lot is the record that applies to the material in your vial.
What purity does B12 have?
Purity is measured by HPLC on every lot and stated on the certificate of analysis for that lot, alongside the net content per vial. Look up the lot number printed on your vial on the Lab Reports page to see the figures for your material.
What exactly is in the vial?
10mg of B12 (cyanocobalamin) as a solid.
How should the vial be stored when it arrives?
Move the sealed vial into freezer storage at −20°C or below, kept dry and protected from light. Allow it to reach room temperature before opening so that moisture does not condense on the solid.
What should I do if the lot number does not match the certificate?
Do not use the material. Contact us with the lot number from the vial label and your order number, and we will resolve it before any work goes ahead.
Who can purchase B12?
Sale is restricted to persons aged 21 or over who are qualified researchers or who purchase on behalf of a research institution, laboratory or business. The material is supplied for laboratory research only and not for use in humans or animals.
Looking for something else?
Tell us the compound and quantity you are looking for and we will confirm availability and lead time. Research use only.
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